Brassica carinata A. Braun, commonly known as Ethiopian mustard or Abyssinian mustard, is an allotetraploid species with increasing agricultural interest due to its significant potential as a biodiesel feedstock. Traditionally grown as a leafy vegetable and oilseed crop in the Ethiopian highlands, attention has recently centered on the expansion of B. carinata cultivation due to its fatty acid composition and numerous favorable agronomic characteristics, such as pod shatter resistance and disease tolerance (Raman et al., 2017). This highly heat- and drought-tolerant species has now spread as a winter or rotational with wheat and other cereals in North America, Europe, Australia, and Asia (Marillia et al., 2014). B. carinata is a multipurpose crop species best suited for non-food applications due to its seeds’ high erucic acid content, which is toxic for human consumption (Husen et al., 2014). However, the oil produced by the seeds can be used to make biodiesel, biopolymers, and biolubricants, and the seed meal has a high protein content. The plant is also useful for biopesticides, antioxidants, and phytoremediation of heavy metal contamination from soils (Mourato et al., 2015; Thakur et al., 2019). In terms of biodiesel use, the fatty acid composition of B. carinata is considered superior as it avoids oxidation during long-term storage. Additionally, the defatted protein byproduct has a higher protein content than that of B. napus and can be used for animal and fish fodder (Marillia et al., 2014; Thakur et al., 2019).